N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin
Thiazovivin
CAS: 1226056-71-8
Molecular Formula: C15H13N5OS
N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin - Names and Identifiers
N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin - Physico-chemical Properties
| Molecular Formula | C15H13N5OS |
| Molar Mass | 311.36 |
| Density | 1.379 |
| Solubility | DMSO: soluble20mg/mL, clear |
| Appearance | powder |
| Color | , white to beige to brown |
| Storage Condition | -20°C |
| Stability | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 2 months. |
| In vitro study | Despite having little effect on cell proliferation, Thiazovivin treatment significantly enhanced the survival of human embryonic stem cells (hESCs) by more than 30 times after enzymolysis, at the same time, the pluripotency of unique colony morphology, and the expression of typical pluripotency markers, such as alkaline phosphatase (ALP), and normal karyotype, are uniformly maintained. Treatment of dissociated hESCs with Thiazovivin significantly increased their adhesion to artificial basement membrane-or laminin-coated plates within a few hours, while there was no effect on gelatin-coated plates. Thiazovivin treatment increased cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrins, Thiazovivin, but not Tyrintegin (Ptn), is also able to mediate cell-cell interactions through E-cadherin, protect hESCs from death in ECM-deficient suspensions. Thiazovivin treatment effectively inhibits E-Cadherin Endocytosis, thereby stabilizing cell-surface E-cadherin and restoring cell-cell interactions, this is necessary for hESC to survive in an ECM-free environment. Thiazovivin, but not Tyrintegin (Ptn), inhibits Rho-associated kinase (ROCK) activity at a concentration of 2 μm and protects hESCs, similar to the effect of the widely used selective ROCK Inhibitor Y-27632 at a concentration of 10 μm, Rho-ROCK signaling is shown to regulate cell-ECM and cell-cell adhesion. 1 μm Thiazovivin increased the recombination potency of CB monocytes to induce more than 10-fold pluripotent stem cells (iPSCs). |
N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | 22 - Harmful if swallowed |
| WGK Germany | 3 |
N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.212 ml | 16.059 ml | 32.117 ml |
| 5 mM | 0.642 ml | 3.212 ml | 6.423 ml |
| 10 mM | 0.321 ml | 1.606 ml | 3.212 ml |
| 5 mM | 0.064 ml | 0.321 ml | 0.642 ml |
Last Update：2024-01-02 23:10:35
N-(Phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide Thiazovivin - Reference Information
| biological activity | Thiazovivin is a novel ROCK Inhibitor with an IC50 of 0.5 μm, promotes the survival of human embryonic stem cells (hESCs). Thiazovivin, a novel ROCK Inhibitor with an IC50 of 0.5 μm in a cell-free assay, promotes the survival of human embryonic stem cells (hESCs) after single cell isolation. |
| Target | Value |
| ROCK (Cell-free assay) | ~0.5 μM |
Last Update：2024-04-10 22:29:15
Supplier List
Spot supply
Product Name: Thiazovivin Visit Supplier Webpage Request for quotationCAS: 1226056-71-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Spot supply
Product Name: Thiazovivin Visit Supplier Webpage Request for quotationCAS: 1226056-71-8
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
View History